Lasers for flow cytometry
Low noise, narrow linewidth DPSS lasers for high-throughput cytometry
Skylark Lasers' continuous-wave single frequency lasers are designed to meet the demanding needs of advanced flow cytometry. From high-parameter immunophenotyping to industrial-scale cell sorting, Skylark NX lasers provide repeatable and reliable UV excitation for dyes and assays that cannot be addressed with visible or near-IR sources.
Uniform intensity
≤ 0.3% RMS
Low intensity noise provides steady excitation for high signal-to-noise fluorescence detection and accurate, reliable gating.
Stable power
≤ 2.0%
Minimal intensity drift across typical operating temperatures maintains signal fidelity and throughput in continuous sorting.
Uniform beam
M² ≤ 1.2
Consistent wavelength
± 0.2 pm
Stable wavelength over extended operation, ensuring consistent fluorophore excitation and accurate fluorescence ratio measurement.
UV lasers for advanced flow cytometry applications
Flow cytometry relies on precise excitation sources to enable sensitive detection and reproducible analysis of cells and particles.
For applications at 320 nm, 349 nm, 780 nm, and 785 nm, Skylark NX single frequency CW DPSS lasers provide the stability and spectral quality required for demanding assays.
Narrow linewidth, frequency stability, spectral purity, and wavelength accuracy are critical for minimising background noise, generating efficient fluorophore excitation, and maintaining compatibility with advanced optical filtering strategies. These characteristics directly impact signal clarity, population resolution, and the reproducibility of cytometric data.
Skylark Lasers CW DPSS series of lasers are designed for high throughput flow cytometry applications, supporting assays that demand high sensitivity in the UV with 320 nm or 349 nm for DNA and protein quantification, as well as in the NIR with 780 nm or 785 nm for viability dyes, scattering applications, and multiplexed detection.
Leverage single frequency operation and improved signal-to-noise for high confidence in your quantitative cytometry results across a range of flow cytometry techniques.
DNA content and cell cycle analysis
For flow cytometry applications such as DNA quantification and cell cycle analysis, 349 nm CW single frequency DPSS lasers provide ideal excitation for Hoechst and DAPI dyes. At 349 nm, these dyes are strongly excited, producing bright, reproducible signals with low CVs and high sensitivity. The low noise, stable output power, and narrow linewidth of single frequency DPSS lasers contribute to accurate DNA histograms and precise cell cycle profiling with consistent results for research and clinical cytometry applications.
Side population (SP) analysis
Skylark 349 NX flow cytometry lasers deliver the high beam quality and pointing stability required for reliable Hoechst dye excitation in side population assays, enabling the detection of small differences within cell populations. Delivering long term stability, the 349 NX delivers consistent excitation during high speed cell sorting, improving resolution and reproducibility compared to pulsed UV lasers.
Sperm and ploidy analysis
High-throughput ploidy and sperm DNA content analysis applications such as sex-sorting and gamete characterisation depends on precise, stable UV excitation to resolve subtle fluorescence intensity differences between cell populations.
Compared with traditional pulsed UV or HeCd laser sources, Skylark NX 349 nm lasers deliver a stable, low noise beam output with up to 400 mW for consistent excitation at high flow rates.
Continuous wave operation eliminates pulse-to-pulse intensity variation, improving fluorescence ratio precision and measurement repeatability. Narrow linewidth and long term wavelength stability ensures reliable discrimination and accurate gating across extended sorting sessions.
Skylark Lasers deliver stable excitation for high-throughput flow cytometry
Skylark NX lasers combine efficient high power with spectral purity — even at the fiber output. Delivering ultra-stable output with an ultra-narrow linewidth, Skylark NX lasers at 780 nm and 785 nm provide a clean beam with low ASE noise < – 80 dB without filters or mode cleaning.
Low noise
Providing cleaner data acquisition and improved separation of cell populations.
Excellent beam quality
Inherently clean, low noise beam delivering high spectral purity for efficient transmission through cytometer optics while maximising fluorescence signal.
True single frequency output
Ultra-stable wavelength and output power intensity to reduce measurement fluctuations, ensuring consistent excitation and reproducible results in flow cytometry.
Narrow linewidth
Enabling precise fluorophore excitation while minimising unwanted background signals.
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